Day by day, the technology of various knowledge is progressing. One of these sciences is biology. which has undergone a lot of development and progress in recent times. In this science, research and study about biological cells is very important. The MTT test is actually a test that examines and analyzes the survival or death or proliferation of cells under different conditions. TrusTrade team is going to investigate this very important and practical test. Stay with us.

A summary of the MTT test ?

The MTT test stands for MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide). In simple terms, the MTT test is a simple method to check cell survival under the use of, for example, a drug or substance, and the goal is to determine the effect Check the toxicity of compounds, drugs or other supplements on the cell.

This method is based on colorimetry. In this way, it examines the yellow crystals of tetrazolium. And its changes are expressed by succinate dehydrogenase enzyme as the result of the test.

Forward to the MTT test?

At first, it should be said that this experiment has three stages: cell culture (Seeding), treatment (Treatment) and finally MTT analysis.

It is worth noting that each of these steps takes one day.

The noteworthy point is that this test is one of the most reliable biological and biotechnology tests. One of the most important reasons for the reliability of this method compared to other tests is the high accuracy of this test in distinguishing live from dead cells.

At the beginning, tetrazolium substance, which is the primary and most important substance of this test, is used in powder form along with PBS substance. By combining these two substances, a solution is obtained that is highly sensitive to light and has a yellow color. It is absorbed by the cell membrane with its positive charge. After these steps, this yellow solution enters the cells with the help of endocytosis and goes to the mitochondria.

This substance can be activated in the mitochondria by succinate dehydrogenase in the respiratory cells of the cell, where it turns into purple deposits. In fact, this problem means that if the cells in the device are alive, it is shown and recognizable because of the succinate dehydrogenase enzyme.

To detect the color of the resulting solution, we use a device called a spectrophotometer. This device is able to determine the optical density of living cells at a wavelength of 579 nm with a base wavelength of 630 nm.

To perform the MTT test, regardless of the main device, we need some equipment. An autoclave is needed to sterilize glass containers. A bain-marie is needed to heat the culture medium and open the frozen sample. We use centrifuges to separate the cells. And we also use an inverted optical microscope to see the cells. Also, in the laboratory environment, we use a class II laboratory hood to prevent the spread of odors or toxic gases.

What is the Elisa Reader device ?

Elisa Reader is a device that reads the results of the MTT test. This device is able to determine antibodies in the system directly.

Important points of the MTT test

1. It is better to test 3 times for each cell to reduce the percentage of error.
2. The number of allowed cells in the well should be between 103 and 105 cells.
3. The amount of cells in the well should not be more or less than the stated amount. And it must be counted accurately.
4. We should get the effect time of each drug accurately through articles or research. Because it is very important and vital to do the test.
5. A lot of accuracy and concentration is the most important issue to perform this test as accurately as possible. which must be done in a suitable environment.